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Bartonella spp. Infection in Edible Dormice and Their Fleas
Klimešová, Běla ; Votýpka, Jan (advisor) ; Balážová, Alena (referee)
Bartonellosis is a group of emerging infectious diseases caused by facultatively intraerythrocytic bacteria transmitted between vertebrate hosts by arthropod vectors. Although only some Bartonella spp. are traditionally associated with human diseases, it was proposed that virtually any species can have zoonotic potential. The highest diversity of species was described from rodents acting as reservoirs with variable prevalence reaching up to 90%. Fleas associated with mammalian hosts play an important role in the Bartonella sp. lifecycle and are described to act not only as competent vectors but also as reservoirs for several species. The role of edible dormice (Glis glis), which often interacts with humans, and their fleas in the bartonellae transmission and lifecycle has so far been unknown; therefore, the prevalence and diversity of Bartonella spp. in edible dormice in the Czech Republic were studied. Complementary PCR analysis of four loci (gltA, rpoB, ftsZ, ITS), MALDI- TOF, and cultivation was performed to identify Bartonella sp. infections. The prevalence reaching 90% was found in dormice and their fleas. Altogether, four species were found and several genotypes of B. grahamii-like and yet undescribed Bartonella sp. were cultivated, and their morphology and growth requirements were...
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Fleas of dogs and cats - possibilities of prevention in different geographical conditions
Vodenková, Gabriela ; Lukešová, Daniela (advisor) ; Alica, Alica (referee)
Fleas are worldwide distributed parasites with a capability of transmitting dangerous diseases and causing many other problems to animals and humans. Bachelor thesis was focussed on the possibilities of prevention on flea infestation, these seemingly innocuous insects. Based on available sources of scientific and professional literature were briefly summarized general findings about their biology, furthermore flea-borne diseases were chosen and described, especially dangerous zoonoses. Attention was dedicated to monitoring and factors, which influence their transmission in the evironment. Questionnaire survey was oriented on veterinarians and breeders and evaluating expenditures for prevention and treatment on flea infestation, when at breeders expenditures amounted c. 500,-Kč. Veterinarians was mentioning expenditures for prevention around 800,-Kč and on therapy even 1500,-Kč for dogs and cats treatment in a calendar year. Preference of medicines registered at the Czech market of breeders and veterinarians was congenerous. In the most cases they congruently chose products from farmaceutical companies Bayer, Merial and MSD. Breeders chose antiparasitic collars and spot-on application form, while veterinarians prefered spot-on and peroral tablets. Gained results of the questionnaire survey can help breeders with choosing and application of efficient antiparasitics designated for preclusion of transmission of fleas and ticks of dogs and cats in the evironment.
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